Marker-Assisted Selection for Recognizing Wheat Mutant Genotypes Carrying HMW Glutenin Alleles Related to Baking Quality

Table 2

PCR cycling information for amplifying specific alleles.

Primers	Annealing		Extension		Final extension
	Temp	Time	Temp	Time	Temp	Time
P1P2	64°C	1′	72°C	1′	72°C	6′
P3P4	65°C	1′	72°C	1′	72°C	6′
P5P6	66°C	1′	72°C	1′	72°C	6′
P7P8	58°C	1′	70°C	2.20′′	70°C	7′
P9P10	66°C	1′	70°C	2.30′′	70°C	7′
P11P12	60°C	1.40′′	70°C	2.10′′	70°C	7′
P13P14	61°C	1′	70°C	2.30′′	70°C	7′
P15P16	61°C	1′	70°C	2′	72°C	7′
P17P18	59.5°C	1′	70°C	1′	70°C	7′
P19P20	62°C	50′′	70°C	2′	70°C	7′