A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain

Table 2

Morphological and biochemical identification of S9 bacteriocin producing bacterial isolate.

Test	Result
Cell shape	Rods
Gram stain	+ve
Spore formation	+ve
Growth on MYP	Non-mannitol utilizer
Motility	+ve
Growth at pH 6–8	+ve
Growth at 50^°C	+ve
Growth at 7.5% NaCl	−ve
Starch hydrolysis	+ve
Casein hydrolysis	+ve
Tween 20 hydrolysis	−ve
Nitrate reduction	+ve
Catalase	+ve
Oxidase	+ve
Urease	−ve
Indole production	−ve
Citrate utilization	−ve
Voges-Proskauer (VP)	+ve
Methyl red (MR)	−ve
Gelatin liquefaction	+ve
Hemolysis on sheep blood agar	-Hemolytic
Lecithinase on MYP	+ve
Penicillin resistance	+ve
Oxidative fermentation (OF):
Glucose fermentation	+ve (yellow/blue: O/NF)^*
Galactose fermentation	+ve (yellow/blue: O/NF)
Sucrose fermentation	+ve (yellow/yellow: O/F)^**

O/NF: oxidative/non-fermentative.
^**O/F: oxidative/fermentative.