Research Article

A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain

Table 6

Sensitivity of some indicator strains to BAC YAS 1.

Indicator strainBAC YAS 1 activity (inhibition zone diameter, mm ± SE)*Gram nature

Bacteria
Acinetobacter sp. (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)0.00Gram-negative
Bacillus cereus (environmental strain isolated from soil, Egypt)0.00Gram-positive
B. licheniformis SHG2 (environmental strain isolated from soil, Egypt)13 ± 1.70Gram-positive
B. licheniformis SHG6 (environmental strain isolated from soil, Egypt)0.00Gram-positive
B. licheniformis SHG10 (environmental strain isolated from soil, Egypt)0.00Gram-positive
B. subtilis EMBLAKE (environmental strain isolated from El-Mahmoudia Lake, Egypt)0.00Gram-positive
B. subtilis AS1 (environmental strain isolated from soil, Egypt)10.25 ± 0.48Gram-positive
B. subtilis NCTC 10400/ATCC 66330.00Gram-positive
B. subtilis (environmental strain isolated from soil, Egypt)0.00Gram-positive
Bacillus sp. Ash2 (environmental strain isolated from soil, Egypt)0.00Gram-positive
Campylobacter jejuni NCTC 11322/ATCC 2942814.00 ± 0.41Gram-negative
Clostridium perfringens NCTC 8237/ATCC 1312415.50 ± 1.5Gram-positive
Citrobacter sp. (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)0.00Gram-negative
Enterobacter aerogenes  NCTC10006/ATCC1304315.50 ± 0.46Gram-negative
Enterobacter sp. (human clinical isolate I, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)0.00Gram-negative
Enterobacter sp. (human clinical isolate II, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)0.00Gram-negative
Enterococcus sp. (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)13.75 ± 0.35Gram-negative
E. coli (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)0.00Gram-negative
E. coli NCTC 12241/ATCC 259220.00Gram-negative
Enterococcus faecalis NCTC 12697/ATCC 292120.00Gram-negative
E. amylovora (Department of Plant Pathology, Faculty of Agriculture, University of Alexandria, Egypt)41.00 ± 0.43Gram-negative
Klebsiella sp. (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)29.5 ± 0.4Gram-negative
Lactobacillus bulgaricus (dairy product isolate, Department of Dairy Products, Faculty of Agriculture, University of Alexandria, Egypt)0.00Gram-positive
L. casei (dairy product isolate, Department of Dairy Products, Faculty of Agriculture, University of Alexandria, Egypt)0.00Gram-positive
L. lactis (dairy product isolate, Department of Dairy Products, Faculty of Agriculture, University of Alexandria, Egypt)0.00Gram-positive
L. reuteri (dairy product isolate, Department of Dairy Products, Faculty of Agriculture, University of Alexandria, Egypt)0.00Gram-positive
Listeria innocua NCTC 11288/ATCC 3309037.75 ± 0.49Gram-positive
Pseudomonas aeruginosa strain EGY II0.00Gram-negative
P. aeruginosa (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)0.00Gram-negative
Proteus sp. (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)14.00 ± 0.3Gram-negative
Salmonella typhimurium NCTC 12023/ATCC  1402817.25 ± 0.49Gram-negative
S. typhi (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)0.00Gram-negative
S. paratyphi (human clinical isolate, Department of Microbiology, Faculty of Medicine, University of Alexandria, Egypt)0.00Gram-negative
Shigella boydii ATCC 92070.00Gram-negative
Staphylococcus aureus NCTC 12981/ATCC 259230.00Gram-positive
Staphylococcus epidermidis NCTC 13360/ATCC 1222823.75 ± 0.75Gram-positive
Vibrio parahaemolyticus NCTC 31780.00Gram-negative
Fungi and yeast
Aspergillus brasiliensis NCPF 2275/ATCC 164040.00
Candida albicans NCPF 3179/ATCC 102310.00
C. tropicalis (human clinical isolate from diabetic foot, Department of Microbiology, Medical Research Institute, University of Alexandria, Egypt)0.00
Saccharomyces cerevisiae NCPF 31780.00

Mean of four readings with standard error (SE).