Derivation and Characterization of a ES-Like Cell Line from Indian Catfish Heteropneustes fossilis Blastulas

Table 2

Details of the primers used for the amplification of mtDNA genes.

mtDNA Gene	Primer ref.	Primer Sequence	Primer Ta
COI 655 bp	[23]	FishF1 5′-TGATCCGGCTTAGTCGGAACTGC-3′	50°C for 1 min
		FishR1 5′-GATGTGTGTTGAATTA CGGTCGG-3′
Cyto b 735 bp	[24]	L14841 5′-AGCTTCCATCCAACATCTCAGCATG ATGA	50°C for 1 min
		H15149 5′-ACTGCAGCCCCTCAGAATGATATTT GTCCTC A-3′
16S rRNA 485 bp	[25]	L2510 5′-CGCCTGTTTATCAAAAACAT-3′	53°C for 1 min
		H3080 5′-CCGGTCTGAACTCAGATCACGT-3′