Evaluation and Prediction of the HIV-1 Central Polypurine Tract Influence on Foamy Viral Vectors to Transduce Dividing and Growth-Arrested Cells

<table>The structural elements of pWPXL and pMD9 parental plasmid backbones are shown: long terminal repeat (LTR) with U3, R, and U5 regions, ref-responsive element (RRE), human elongation factor alpha promoter (EF1-<svg height="7.9499998" id="M1" style="vertical-align:-0.1254pt" version="1.1" viewbox="0 0 9.7749996 7.9499998" width="9.7749996" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M545 106q-67 -118 -134 -118q-24 0 -40 37.5t-30 129.5h-2q-47 -72 -103 -119.5t-108 -47.5q-47 0 -76 45.5t-29 119.5q0 113 85 204t174 91q47 0 70 -33.5t43 -119.5h3q32 47 80 140l55 13l10 -9q-47 -80 -138 -201q17 -99 27.5 -136t22.5 -37q23 0 69 61zM333 204
q-14 98 -31 149.5t-50 51.5q-49 0 -94 -70t-45 -164q0 -55 15.5 -86t40.5 -31q70 0 164 150z" id="x1D6FC"></path></g>
</svg>), gene encoding enhanced green fluorescent protein (eGFP), enhancer/promoter of the human cytomegalovirus immediate early gene (CMV),<i> cis</i>-acting sequences (CASI and CASII), constitutively active spleen focus forming virus U3 promoter (SFFV U3), and internally deleted U3 region of the 3′ LTR (ΔU3). Foamy viral cPPT was replaced with cPPT (HIV) in different variations. CTS was deleted or inserted in accordance with the scheme shown above.</table>

The Scientific World Journal

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Figure 2

Figure 2: Evaluation and Prediction of the HIV-1 Central Polypurine Tract Influence on Foamy Viral Vectors to Transduce Dividing and Growth-Arrested Cells 

Figure 2 | Evaluation and Prediction of the HIV-1 Central Polypurine Tract Influence on Foamy Viral Vectors to Transduce Dividing and Growth-Arrested Cells 