Vitamin C Protected Human Retinal Pigmented Epithelium from Oxidant Injury Depending on Regulating SIRT1

Figure 2

Vit C resulted in the dysregulation of SIRT1, p53, and FOXO3 during oxidative stress in ARPE-19 cells. ARPE-19 cells were treated with indicated methods. The expression of SIRT1 (a), p53 (b), and FOXO3 (c) mRNA was detected by qRT-PCR. Ct values were normalized by 2^−ΔΔCt method as relative quantification. Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statistical significance was expressed as ^∗𝑃<0.05 and ^∗∗𝑃<0.01.

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