Virulence Genotyping of Pasteurella multocida Isolated from Multiple Hosts from India

Table 2

Details of primers and citations used for the detection of capsular type and virulence associated genes in strains of Pasteurella multocida.

Gene	Primer	Primer sequence (5′-3′)	Reference
PM-PCR and Capsular serotypes
KMT1	PMPCR-F; PMPCR-R	ATCCGCTATTTACCCAGTGG; GCTGTAAACGAACTCGCCAC	[22]
hyaD-hyaC	capA F; capA R	GATGCCAAAATCGCAGTCAG; TGTTGCCATCATTGTCAGTG	[23]
bcbD	capB F; capB R	CATTTATCCAAGCTCCACC; GCCCGAGAGTTTCAATCC	[23]
dcbF	capD F; capD R	TTACAAAAGAAAGACTAGGAGCCC; CATCTACCCACTCAACCATATCAG	[23]
ecbJ	capE F; capE R	TCCGCAGAAAATTATTGACTC; GCTTGCTGCTTGATTTTGTC	[23]
fcbD	capF F; capF R	AATCGGAGAACGCAGAAATCAG; TTCCGCCGTCAATTACTCTG	[23]
Iron acquisition genes
tbpA	tbpA F; tbpA R	GGACAGTGCATATAACTTGTT; GGACAGTGCATATAACTTGTTTACTA	[32]
hgbA	hgbA F; hgbA R	CATATCGGATCCTTGAAACCAGAGGAAGCAAAAA; GAATCGGAGCTCACGACCTGGTGAGTAAAAACGAT	In-house [33]
hgbB	HgbB F; HgbB R	ACCGCGTTGGAATTATGATTG; CATTGAGTACGGCTTGACAT	[12]
Adhesins
ptfA	ptfA F; ptfA R	AGGATCCATGAAAAAAGCCATTT; GGAGCTCTTATGCGCAAAATCCTG	In-house
pfhA	pfhA F; pfhA R	TAAGCCTATCGGTTCAAGTCG; GATAAATCTACCCCGTCCTCT	In-house
Sialidases
NanB	NanB F; NanB R	GTCCTATAAAGTGACGCCGA; ACAGCAAAGGAAGACTGTCC	[12]
nanH	nanH F; nanH R	CACTGCCTTATAGCCGTATTC; AGCACTGTTACCCGAACCC	[12]
Dermonecrotoxin
ToxA	ToxA F; ToxA R	TCTTAGATGAGCGACAAGG; GAATGCCACACCTCTATAG	[34]