Molten Globule-Like Partially Folded State of Bacillus licheniformis α-Amylase at Low pH Induced by 1,1,1,3,3,3-Hexafluoroisopropanol
Figure 1
Effect of increasing HFIP concentrations on the spectral characteristics of the acid-denatured BLA at pH 2.0 and 25°C, as monitored by (a) measurements, (b) intrinsic fluorescence measurements at 344 nm upon excitation at 280 nm, and (c) ANS fluorescence measurements at 474 nm upon excitation at 380 nm.
