Functional Characterization of Sesquiterpene Synthase from Polygonum minus

Figure 4

Phylogenetic tree of PmSTS with selected terpene synthases from other plants. Seven previously identified TPS subfamilies (Tps-a to Tps-g) were chosen based on Bohlmann et al. [15] and Danner et al. [16]. The Tps-c and Tps-e subfamilies, which are composed of the copalyl diphosphate (cdp) synthases and kaurene synthases and are involved in primary metabolism, were chosen as outgroups. The alignment was performed using the Clustal Omega algorithm. The tree was built using the neighbour joining method and 1000 replicates for bootstrapping. The numbers indicated are the actual bootstrap values of the branches.