Research Article
Aquaporin-1 Expression in Proliferative Vitreoretinopathy and in Epiretinal Membranes
Table 2
Gene symbols, gene name, accession number, primers sequences, amplicon sizes, and primer efficiencies of the tested genes (mean ± S.E.M.,
).
| | Gene symbols | Gene name | Accession number | Primer sequences | Amplicon size (bp) | Primer efficiency (%) |
| | hsAQP1 | Aquaporin-1 | NM_198098 | F: 5′-TGGACACCTCCTGGCTATTG-3′ |
164 |
107 ± 3 | | R: 5′-GGGCCAGGATGAAGTCGTAG-3′ | | hsActa2 | Smooth muscle actin | NM_001141945.1 | F: 5′-GCTATGTGTGAAGAAGAGG-3′ |
171 |
97 ± 1 | | R: 5′-CACGTAGCTGTCTTTTTGT-3′ | | hsGFAP | Glial fibrillary acidic protein | NM_002055.4 | F: 5′-GTCAGAAGGCCACCTCAAGA-3′ |
109 |
96 ± 2 | | R: 5′-CCTGCCTCACATCACATCCT-3′ | | hsATP5B | ATP synthase subunit beta | NM_001686.3 | F: 5′-AGAGGTCCCATCAAAACCAAAC-3′ |
152 |
101 ± 2 | | R: 5′-AAAAGCCCAATTTTGCCACC-3′ | | hsHPRT1 | Hypoxanthine phosphoribosyltransferase 1 | NM_000194.2 | F: 5′-TGGCGTCGTGATTAGTGATG-3′ |
137 |
98 ± 1 | | R: 5′-CTCGAGCAAGACGTTCAGTC-3′ |
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