Efficient Reprogramming of Mouse Fibroblasts to Neuronal Cells including Dopaminergic Neurons

Figure 2

Direct conversion of MEF cells into neural precursor (NP) cells. (a) A schematic diagram for the generation of MEF-derived NP cells. (b) Representative phase-contrast images. Magnification ×200; scale bar is 100 μm. ((c), (d)) Representative immunofluorescence image of differentiated MEF cells stained with Nestin and Sox1 antibodies. Yellow arrows on the above micrograph indicate Sox1-stained cells. Magnification ×200; scale bar is 50 μm. (e) Reprogramming efficiency. Sox1-positive (red bar) and Nestin-positive (green bar) cells per DAPI-positive cells. Single asterisk is 𝑃<0.01. (f) mRNA expression of neural precursor marker genes Nestin, Sox1, and Msi1 by RT-PCR.

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