Efficient Reprogramming of Mouse Fibroblasts to Neuronal Cells including Dopaminergic Neurons

Figure 3

Direct conversion of MEF cells into neurons. (a) A schematic diagram of the generation of MEF-derived neurons. (b) Representative phase-contrast images of neuron-like cells showing bipolar projections. Magnification ×200; scale bar is 100 μm. ((c), (d)) Representative immunofluorescence image of differentiated MEF cells stained with Tuj1 and MAP2 antibodies. Magnification ×200; scale bar is 50 μm. (e) Reprogramming efficiency of the MEF cells into neuronal cells. Tuj1-positive (green bar) and MAP2-positive (red bar) cells per DAPI-positive cells. Single asterisk is 𝑃<0.01. (f) mRNA expression of neuronal cell marker genes Tuj1 and Map2 by RT-PCR.

(a)

(b)

(c)

(d)

(e)

(f)