Efficient Reprogramming of Mouse Fibroblasts to Neuronal Cells including Dopaminergic Neurons

Figure 4

Direct conversion of MEF cells into dopaminergic (DA) neurons. (a) Schematic diagram of the generation of MEF-derived DA neurons. (b) Representative immunofluorescence of the DA neurons stained with Tuj1 and TH antibodies. A yellow arrow indicates DA neurons. Magnification ×200; scale bar is 50 μm. (I, II, and III) Higher magnification insert of B. (c) Reprogramming efficiency of the MEF cells into DA neuron-like cells. Single asterisk is 𝑃<0.01. (d) mRNA expression of various DA neuronal marker genes by RT-PCR.

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