Research Article

Sublethal Heavy Metal Stress Stimulates Innate Immunity in Tomato

Table 1

Effect of CuCl2 on the production of defense and antioxidative enzymes in tomato plants. Values represent mean ± SE of three separate experiments, each in triplicate.

EnzymesControlConcentrations of CuCl2
1 mM 2.5 mM5 mM

Peroxidase (PO) µmol min−1 g−1 protein188.45 ± 14.01c267.96 ± 7.90b334.51 ± 9.23a239.55 ± 9.10b
Polyphenol oxidase (PPO) U min−1 g−1 protein29.44 ± 1.12c32.25 ± 2.08c54.77 ± 2.37a40.855 ± 1.47b
Phenylalanine ammonia-lyase (PAL)
nmol of trans-cinnamic acid min−1 g−1 protein
94.23 ± 4.39d123.55 ± 6.95c165.22 ± 3.12a140.38 ± 3.78b
β-1,3 Glucanase
mol glucose produced min−1 g−1 protein
26.38 ± 2.29d40.1 ± 1.13c53.25 ± 2.34a47.1 ± 1.39b
Catalase (CAT) mmol min−1 g−1 protein6.83 ± 0.30d11.74 ± 0.61c15.09 ± 0.47b17.67 ± 0.56a
Ascorbate peroxidase (APX) mol min−1 g−1 protein0.177 ± 0.021c0.321 ± 0.024b0.408 ± 0.025a0.253 ± 0.014b

Sharing the same letter are not significantly different () using Duncan’s multiple range test.