Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences

Table 7

Assessment of mitotic stability of the constructs.

Transforming plasmid	Colony size	Doubling time in selective media (hrs)	Avg. number of plasmids/cell	% cells with plasmids	% loss of plasmid per generation
pYEp51G wt	+	6.0 ± 0.5	2 ± 1	3 ± 2	9 ± 2
pYEpGA-3	++	2.2 ± 0.3	33 ± 2	58	4 ± 1
pYEpGA-4	++	2.5 ± 0.5	30 ± 2	47	3 ± 2
pYEpGA-5	+	4.3 ± 0.4	19 ± 2	34	5 ± 2
pYEpGA-6	++	2.0 ± 0.3	2 ± 1	47	4 ± 1
pYEpGA-7	++	3.3 ± 0.2	6 ± 2	40	8 ± 1
pYEpGA-8	+	2.7 ± 0.2	19 ± 1	49	4 ± 2
pYEpGA-9	+++	3.0 ± 0.2	9 ± 2	42	7 ± 2
pYEpGA-12	++	2.0 ± 0.2	34 ± 2	60	4 ± 1
pYEpGA-13	++	2.2 ± 0.3	33 ± 3	62	3 ± 2