Granulocyte-Colony Stimulating Factor Increases Cerebral Blood Flow via a NO Surge Mediated by Akt/eNOS Pathway to Reduce Ischemic Injury

Figure 3

Activation of eNOS and nNOS in rats’ striatal tissues during ischemia. (a) Representative changes of striatal phospho-eNOS (Ser1177) and phospho-nNOS (Ser1416) at 15, 30, and 60 minutes after ischemia in normal (sham control), I/R, and I/R + G-CSF treated rats. β-actin was loaded as internal quality control and used for normalization in densitometric analyses. Quantitative densitometry analysis showing relative expression of phospho-eNOS (b) and phospho-nNOS (c) as fold of sham control. and versus I/R, and versus sham control at 15, 30, and 60 min, respectively.

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