Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production

Figure 3

Effect of tungstate on total and neutral lipid accumulation in D. tertiolecta. (a) Lipid content (histogram) and lipid productivity per cycle (squares). (b) Neutral lipids droplets accumulation in D. tertiolecta stained with Nile red dye. (c) Neutral lipid analysis by spectrofluorimetry expressed as µg of neutral lipids/millions of cells. All cultures were cultivated in Guillard F/2 medium. Tungstate (10 mM) was added to the cultures at the beginning of culture one-stage treatment or at 7th day ‘two-stage treatment’. Data are mean ± standard deviation.

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