Fecal Proteomic Analysis in Healthy Dogs and in Dogs Suffering from Food Responsive Diarrhea
Table 1
Identification of significantly changed fecal proteins from healthy dogs and dogs suffering from food responsive diarrhea (FRD).
Spot
Mr. (kDa) / (±SD)
Normalized quantity Healthy (x103
Normalized quantity FRD (x103
Protein
Score
Mr. (kDa) /
Accession number
A
12.4±0.4/7.9±0.2
550±61
17921
Hemoglobin subunit beta
16∗
Bos Taurus
LLVVYPWTQR
15.9/7.01
gi27819608
I
14.6±0.9/8.70.3
161±23
422±102
Hypothetical protein
19∗
Streptomyces sp.
PAAAAGTAVQ
14.1/9.5
WP_046507073.1
K
17.3±0.7/ 9.5±0
359 ± 130
58± 21
Putative Cytochrome P450
§
Oryza sativa
RTLVVSTAAAAAD LYR
54.8/ 9.1
gi50725157
K1
17.1±0.2/9.8±0
0
126 ± 113
UDP-N-acetylglucosamine diphosphorylase
18∗
Zea mays
RIPSVHGYTSGLK
20.5/8.5
ONM18096.1
L
22.3±0.8/5.1±0.2
0
386±79
Isopentenyl-diphosphate delta-isomerase
31∗
Streptomyces sp.
QSGPRPFDPQEVA
21.1/5.3
WP_073935025.1
O1
15.0±0.7/4,7±0.2
0
118±17
not found
-
O2
14.8±0.7/ 4.9±0.2
0
27.3±17.1
Immunoglobulin J chain isoform 1
§
Canis lupus familiaris
IIPSPDDPNE IVER
18.1/4.7
gi57095596
O3
14.9±0.9/5.2±0.1
0
101±47
not found
-
O4
13.7±0.6/5.6±0.1
0
113±32
not found
-
S
11.4±0.4/5.7±0.1
36±19
94±46
DTW domain-containing protein
19∗
Microbulbifer donghaiensis
KTNTGALALAQCGNLVER
17.4/5.7
WP_073272148.1
T
9.9±0.8/6.2±0.1
60±25
197±95
hypothetical protein U973_01647
19∗
Staphylococcus aureus
IAKGLETAINAINE
10.6/5.8
EZW68888.1
U
44.8±1.3/5.3±0.2
148±41
528±155
Coproporphyrinogen III oxidase
33∗
Pontibacillus chungwhensis
EPVHYKEEMEEQ
44.6/5.6
WP_036785728.1
spot ID as indicated in Figure 1. values calculated from the 2-DE maps by the PDQuest software. (∗) and SONAR (§) results (SwissProt & NCBInr databases).
