Anticancer Potential of Fruit Extracts from Vatica diospyroides Symington Type SS and Their Effect on Program Cell Death of Cervical Cancer Cell Lines

Figure 3

Apoptosis induction effect of cotyledon-acetone extract (ACE) at half IC₅₀, IC₅₀, and 2-fold IC₅₀ on HeLa (a) and SiHa (b) cells for 48 hours determined by Annexin V-FITC/PI flow cytometry. Dot plot diagrams show percentage of cell populations divided to viable (lower left quadrant; Annexin V and PI negative), early apoptotic (lower right quadrant: Annexin V positive and PI negative), late apoptotic (Upper right quadrant; Annexin V and PI positive), and dead cells (Annexin V negative and PI positive).