Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium

Table 3

Metabolic activity of human normal and cancer cells in vitro in culture media with different deuterium contents. Alamar blue assay was performed after 24 h and 72 h of cell cultivation in control or experimental growth media (the results are expressed as mean ± SD, n = 5, compared to the control group).

Exposure time (h)	24			72
Culture medium (%)	Control	DDW	D2O	Control	DDW	D2O
Metabolic activity of А549 cell line	100 ± 3	108 ± 6	69 ± 7	100 ± 4	105 ± 5	69 ± 14
Metabolic activity of HT29 cell line	100 ± 5	105 ± 3	72 ± 6	100 ± 6	107 ± 7	65 ± 11
Metabolic activity of U87 cell line	100 ± 3	110 ± 5	—	100 ± 5	108 ± 11	—
Metabolic activity of hADSCs	100 ± 2	162 ± 11	81 ± 8	100 ± 7	132 ± 13	69 ± 12
Metabolic activity of hDFB	100 ± 5	121 ± 7	—	100 ± 4	115 ± 8	—