Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium

Table 4

Viability of human normal and cancer cells in vitro in culture media with different deuterium contents. FDA/PI assay was performed after 24 h and 72 h of cell cultivation in control or experimental growth media (the results are expressed as mean ± SD, n = 5, compared to the control group).

Exposure time (h)	24			72
Culture medium (%)	Control	DDW	D2O	Control	DDW	D2O
Viability of U87 cell line	100 ± 5	94 ± 5	95 ± 7	100 ± 8	92 ± 7	91 ± 5
Viability of hADSCs	100 ± 11	96 ± 10	75 ± 13	100 ± 12	88 ± 7	82 ± 9
Viability of hDFB	100 ± 8	98 ± 6	83 ± 8	100 ± 9	94 ± 4	85 ± 5