Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium

Table 5

Viability of human normal and cancer cells in vitro in culture media with different deuterium content. Trypan blue staining was performed after 24 h and 72 h of cells cultivation in control or experimental growth media (the results are expressed as mean ± SD, n = 5, compared to the control group).

Exposure time (h)	24			48
Culture medium (%)	Control	DDW	D2O	Control	DDW	D2O
Viability of А549 cell line	100 ± 5	108 ± 12	103 ± 7	100 ± 9	105 ± 8	107 ± 9
Viability of HT29 cell line	100 ± 3	105 ± 6	104 ± 9	100 ± 8	107 ± 5	106 ± 12
Viability of U87 cell line	100 ± 7	102 ± 4	—	100 ± 11	108 ± 7	—
Viability of hADSCs	100 ± 8	110 ± 10	108 ± 12	100 ± 6	102 ± 4	110 ± 11
Viability of hDFB	100 ± 11	109 ± 13	107 ± 12	100 ± 7	111 ± 9	104 ± 7