The Impact of Zinc Oxide Nanoparticles on Cytotoxicity, Genotoxicity, and miRNA Expression in Barley (Hordeum vulgare L.) Seedlings

Table 3

The results of H. vulgare L. seedling RAPD analysis.

Primer ID	Primer sequences (5′-3′)	Length (bp)	Number of polymorphic bands of 1 mg/L		Number of polymorphic bands of 2 mg/L		Number of polymorphic bands of 4 mg/L		Total number of bands
			a	b	a	b	a	b
CB-21	CAGCACTGAC	10	3	3	2	3	3	3	9
OPA-02	TGCCGAGCTG	10	3	2	0	1	4	5	11
OPA-05	AGGGGTCTTG	10	0	0	1	1	1	1	4
OPA-11	CAATCGCCGT	10	0	0	0	0	0	1	3
OPD-18	GAGAGCCAAC	10	0	0	0	2	7	5	15
Total polymorphic bands			6	5	3	7	15	15
Average number of polymorphic bands per experimental group			2.2		2		6

Primers used, number of polymorphic bands in plants treated with 1 mg/L, 2 mg/L, and 4 mg/L of ZnO NPs, total number of bands for each primer, and average number of polymorphic bands for every plant group. a: new band; b: disappeared band.