Detection of <i>Salmonella enterica</i> subsp. <i>enterica</i> via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification

<div>(a) LAMP primer design. Six distinct regions are designated on the target DNA, labeled F3, F2, F1, B1c, B2c, and B3 from the 5′ end. The F1c sequence is complementary to the F1 sequence. Two inner primers (FIP and BIP) and two outer primers (F3 and B3) are used in the LAMP method. FIP (BIP) is a hybrid primer consisting of the F1c (B1c) sequence and the F2 (B2) sequence. (b) LAMP primer mapping. The portion of the SopD gene that is targeted by the developed <i>Salmonella enterica</i> QUASR LAMP assay is shown here. The colored nitrogenous bases show the corresponding LAMP primer in Figure <a href="../fig2/#a">2(a)</a>.</div>

The Scientific World Journal

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Figure 2

Figure 2: Detection of <i>Salmonella enterica</i> subsp. <i>enterica</i> via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification 

Figure 2 | Detection of Salmonella enterica subsp. enterica via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification 