Research Article

Detection of Salmonella enterica subsp. enterica via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification

Table 4

In silico PCR amplification of SopD F3 and B3 primers. In silico PCR amplification was done against different bacterial genomes using a freely available molecular biology resource for experiments (http://insilico.ehu.es/). Interpretation of results are as follows: (+) – Amplification was observed, (−) – No amplification was observed.

OrganismSopD F3 and B3 amplification (+/−)

Escherichia coli
Klebsiella pneumoniae
Staphylococcus epidermidis
Staphylococcus aureus
Bacillus cereus
Enterococcus faecalis
Enterococcus faecium
Listeria monocytogenes
Streptococcus agalactiae
Streptococcus pneumoniae
Streptococcus pyogenes
Haemophilus influenzae
Pseudomonas aeruginosa
Acinetobacter baumannii
Neisseria meningitidis
Bacteroides fragilis
Bacillus anthracis
Yersinia pestis
Francisella tularensis
Salmonella bongori
Salmonella enterica subsp. arizonae serovar 62: z4, z23:--
Salmonella enterica subsp. enterica serovar Cubana+
Salmonella enterica subsp. enterica serovar Agona+
Salmonella enterica subsp. enterica serovar Bareilly+
Salmonella enterica subsp. enterica serovar Bovismorbificans+
Salmonella enterica subsp. enterica serovar Choleraesuis+
Salmonella enterica subsp. enterica serovar Dublin+
Salmonella enterica subsp. enterica serovar Enteritidis+
Salmonella enterica subsp. enterica serovar Gallinarum+
Salmonella enterica subsp. enterica serovar Gallinarum/pullorum+
Salmonella enterica subsp. enterica serovar Heidelberg+
Salmonella enterica subsp. enterica serovar Javiana+
Salmonella enterica subsp. enterica serovar Newport+
Salmonella enterica subsp. enterica serovar Paratyphi A+
Salmonella enterica subsp. enterica serovar Paratyphi B+
Salmonella enterica subsp. enterica serovar Paratyphi C+
Salmonella enterica subsp. enterica serovar Pullorum+
Salmonella enterica subsp. enterica serovar Schwarzengrund+
Salmonella enterica subsp. enterica serovar Thompson+
Salmonella enterica subsp. enterica serovar Typhi+
Salmonella enterica subsp. enterica serovar Typhimurium+