Fractionation and Antibacterial Evaluation of the Surface Compounds from the Leaves of Combretum zeyheri on Selected Pathogenic Bacteria

Table 2

Antibacterial activities of the fraction separated by chromatography and pooled based on the TLC profile.

Pool number	TLC fraction	Pooled fraction name	% inhibition of growth of S. aureus; MIC/MBC^L; MIC/MBC^C	% inhibition of growth of P. aeruginosa; MIC/MBC^L; MIC/MBC^C
Pool 18	111-112	CZSC111112	51	71
Pool 19	113–120	CZSC113120	29	70
Pool 20	121–131	CZSC121131	31	67
Pool 21	133–139	CZSC133139	++	68
Pool 22	141–146	CZSC141146	++	++
Pool 23	147–149	CZSC147149	++	++
Pool 24	151–154	CZSC151154	++ (25/25); (>100/>100)	++ (50/50); (50/100)
Pool 25	155–160	CZSC155160	++ (25/25); (>100/>100)	++ (25/25); (25/50)
Pool 26	161–166	CZSC161166	++	++
Pool 27	168–172	CZSC168172	++	++
Pool 28	173-174	CZSC173174	++	++
Pool 29	175-176	CZSC175176	++	++
Pool 30	177-178	CZSC177178	++	++
Pool 31	179-180	CZSC179180	++	++
Pool 32	182-183	CZSC182183	++	++
Pool 33	186-187	CZSC186187	++	++
Pool 34	189–194	CZSC189194	++	++

—, no inhibition of growth of the test pathogen. ++, complete inhibition of growth of the test pathogen. ND, the activities of these fractions were not determined since they were in small amounts. MIC/MBC^PaL, values for the lab strain of P. aeruginosa ATCC 27853. MIC/MBC^PaC, values for the clinical strain of P. aeruginosa. MIC/MBC^SaL, values for the lab strain of S. aureus ATCC 9144. MIC/MBC^SaC, values for the clinical strain of S. aureus.