Fractionation and Antibacterial Evaluation of the Surface Compounds from the Leaves of Combretum zeyheri on Selected Pathogenic Bacteria

Table 3

Antibacterial activities of the fraction separated by chromatography and pooled based on the TLC profile.

Pool number	TLC fraction	Pooled fraction name	% inhibition of growth of S. aureus; MIC/MBC^L; MIC/MBC^C	% inhibition of growth of P. aeruginosa; MIC/MBC^L; MIC/MBC^C
Pool 35	197-198	CZSC197198	ND	++
Pool 36	199-200	CZSC199200	ND	++
Pool 37	203–206	CZSC203206	++	++
Pool 38	207-208	CZSC207208	++	++
Pool 39	209–213	CZSC209213	++ (12.5/12.5); (25/>100)	++ (12.5/12.5); (12.5/12.5)
Pool 40	214-215	CZSC214215	++	++
Pool 41	216–219	CZSC216219	++	++
Pool 42	221-222	CZSC221222	++	++
Pool 43	223–234	CZSC223234	++	++
Pool 44	236–241	CZSC236241	++	++
Pool 45	243-244	CZSC243244	++	++
Pool 46	246–259	CZSC246259	++	++
Pool 47	260-261	CZSC260261	++	++

—, no inhibition of growth of the test pathogen. ++, complete inhibition of growth of the test pathogen. ND, the activities of these fractions were not determined since they were in small amounts. MIC/MBC^PaL, values for the lab strain of P. aeruginosa ATCC 27853. MIC/MBC^PaC, values for the clinical strain of P. aeruginosa. MIC/MBC^SaL, values for the lab strain of S. aureus ATCC 9144. MIC/MBC^SaC, values for the clinical strain of S. aureus.