MC1R Gene Variants and Their Relationship with Coat Color in South American Camelids

Table 1

Primers used to amplify the coding region of the MC1R gene and 5′ and 3′ regions.

Amplicon	Primer	Location
AMPL2094177	Fw: CCACAGCTCTTCCTGGAGATTC; Rv: CTGCATCTTCAAGAATTTCAACCTCT	137308,137330; 137602,137628
AMPL2094179	Fw: GCCAGCATGTGGACATATAGCA; Rv: ACGTCATGGATGTGCTCATCTG	137815,137837; 138075,138097
AMPL2094181	Fw: AGGCAGCAGATGAAGTAATACATGG; Rv: CCAGGGAGAAGGTGAGTGTGA	138211,138236; 138525,138546
AMPL2094180	Fw: GGTAGCGCAGTGCATAGAAGAT; Rv: CTGCCATCACCAAGAACCGCAA	137993,138015; 138246,138268
AMPL2094178	Fw: CCACGATGGAGTTGCAGATGAT; Rv: CTCCTCTGTCTCGTCAGCTTTTT	137570,137592, 137862,137885

All the primers were using the reference (GCF_000164845.3). Vicugna_pacos 3.1. Primary assembly. AMPL: amplicon; Fw: forward strand; Rv: reverse strand.