Molecular Identification and Antioxidant Activity Determination among Coffee Varieties Cultivated in Nepal

Table 2

Pairwise comparisons of ITS2 sequences obtained from the nine coffee trees listed in Table 1.

SN		1	2	3	4	5	6	7	8	9
1	Sankhuwasabha_Red
2	NARC-C5	0.0000
3	Syangja_Special	0.0046	0.0031
4	NARC-C4	0.0015	0.0000	0.0060
5	NARC-C3	0.0015	0.0000	0.0060	0.0015
6	NARC-C2	0.0015	0.0000	0.0060	0.0015	0.0000
7	NARC-C1	0.0015	0.0000	0.0090	0.0075	0.0000	0.0000
8	Kshetradeep	0.0000	0.0000	0.0046	0.0015	0.0030	0.0030	0.0030
9	Gulmi_Local	0.0015	0.0000	0.0060	0.0015	0.0000	0.0000	0.0000	0.0030

The number of base substitutions per site between sequences is shown. Analyses were conducted using the Jukes–Cantor model [29]. The rate variation among sites was modeled using a gamma distribution (shape parameter = 1).