Ophthalmomyiasis Case Caused by Two Blow Fly (Diptera: Calliphoridae) Species in North America
Table 1
Taxonomic assignment of unknown larvae using DNA-based (whole mitochondrial genome and COI barcode region) and morphological approaches.
Sample #
Larval attributes
Taxonomic assignment
Instar
Specimen length (mm)
Whole mitochondrial genome
COI barcode region
Morphology
Sequence length (bp)
Query coverage (%)
Identity (%)
E-value
Taxonomic assignment
Query coverage (%)
Identity (%)
E-value
Taxonomic assignment
Taxonomic assignment
1
2nd
4
14,906
100
99.93
0.0
Phormia regina
100
100
0.0
Phormia regina
Unknown
2
3rd
4
14,893
100
99.97
0.0
Lucilia coeruleiviridis
100
100
0.0
Lucilia sp.
Lucilia coeruleiviridis
3
3rd
2
14,906
100
99.92
0.0
Phormia regina
100
100
0.0
Phormia regina
Unknown^
4
3rd
6
14,893
100
99.99
0.0
Lucilia coeruleiviridis
100
100
0.0
Lucilia sp.
Lucilia coeruleiviridis
5
3rd
5
14,893
100
99.97
0.0
Lucilia coeruleiviridis
100
100
0.0
Lucilia sp.
Lucilia coeruleiviridis
Match statistics from GenBank blastn searches are given. bp, base pair; COI, cytochrome oxidase subunit I. based on posterior two-thirds of the specimen. ^only taxonomically assigned to the family level (Calliphoridae).
