Molecular Detection and Phylogenetic Analysis of the alkB Gene in Klebsiella oxytoca Strains Isolated from the Gut of Tenebrio molitor

Figure 2

Amplification of the three Klebsiella oxytoca strains with JCM-specific primers on 1.5% agarose gel. Lane 1:100 bp ladder, lane 2: sample 1 which was identified as Klebsiella oxytoca JCM 1665, lane 3: positive control which is the master mix with 16S ribosomal RNA primers plus the DNA template, lane 4: negative control which is the PCR master mix without the DNA template, and lanes 5 and 6 had samples 2 and 3, respectively.