Molecular Detection and Phylogenetic Analysis of the alkB Gene in Klebsiella oxytoca Strains Isolated from the Gut of Tenebrio molitor

Table 3

Forward and the reverse primer sequences designed to amplify the 16S ribosomal RNA of the Klebsiella oxytoca NBRC 102593 strain, Klebsiella oxytoca JCM 1665 strain, and Klebsiella oxytoca ATCC 13182 strain and alkB gene in Klebsiella oxytoca strains.

Target	Primer sequence (5′-3′)	(°C)	GC (%)	Amplicon size (bp)
Klebsiella oxytoca NBRC 102593	NBRC forward: GAT​GAC​CAG​CCA​CAC​TGG​AA	59.96	55	247
	NBRC reverse: GCT​GCA​CCC​TCC​GTA​ATA​CC	60.53	60
Klebsiella oxytoca ATCC 13182	ATCC forward: TCT​CGG​GTG​ACG​AGT​GTT​A	57.67	52.63	329
	ATCC reverse: CTT​CAT​ACA​CGG​CGG​CAA​TG	59.97	55
Klebsiella oxytoca JCM 1665	JCM forward: CCA​GCC​ACA​CTG​GAA​CTG​AG	60.6	60.0	262
	JCM reverse: TCC​CCG​GAT​TTA​AAC​GGC​TTT​G	61.46	50.0
alkB gene	Deg-f forward: CACTTCTACRTCGATCAC	42.81	50	338
	Deg-r reverse: CCRTAGTGCTCGAGGTAG	47.69	58.82