Research Article

Leaf Extracts of Moringa oleifera Cultivated in Baghdad: Characterization and Antimicrobial Potential against Endodontic Pathogens

Table 2

HPLC conditions for flavonoids.

InstrumentShimadzu, Japan

Mobile phase(A) solution: 1% acetic acid
(B) Solution : acetonitrile : water 80% in ratio of 90% B : A
Particle size5 μm
ColumnODS C18 (250 mm × 4.6 mm internal diameter)
Flow rate1.2 mL/min
Column temperatureRoom temperature
Injection volume20 μL
Injection concentration1 μg/mL
Detection wavelengthUV-Vis at wavelength of 254 nm
Standards usedRutin, caffeic acid, apigenin, hesperetin, kaempferol, coumarin, and myricetin