Isolation and Characterization of Coronavirus and Rotavirus Associated with Calves in Central Part of Oromia, Ethiopia

Table 1

Primer details.

Primer	Primer sequences	Size of amplicons (bp)	Melting temp.	Primer length
VP4-F	TGGCTTCGCTCATTTATAGACA	880	54.2	22
VP4-R	ATTTCGGACCATTTATAACC		47.4	20
BCV-F	GCCGATCAGTCCGACCAATC	407	55	20
BCV-R	AGAATGTCAGCCGGGGTAT		52	19