Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions

Table 2

Results of analysis of variance: significance () of the sources of variation affecting sperm viability and CASA parameters in experiment 1.

Sperm variables^1	Sources of variation^2
	pH	ET	pHET
SMI (%)	ns	ns	ns
TMS (%)	0.0004	0.0196	ns
PMS (%)	0.0096	ns	ns
VCL (μm/s)	0.0003	0.0175	ns
VSL (μm/s)	0.0001	0.0001	ns
VAP (μm/s)	0.0001	0.0004	ns
LIN (%)	0.0003	0.0001	ns
STR (%)	ns	0.0001	ns
WOB (%)	0.0001	0.0003	ns
ALH (μm)	0.0007	0.0001	ns
BCF (Hz)	0.0203	0.0001	ns

¹SMI: sperm membrane integrity; TMS: total motile sperm; PMS: progressive motile sperm; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN = VSL/VCL × 100; STR = VSL/VAP × 100; WOB = VAP/VCL × 100; ALH: amplitude of lateral head displacement; BCF: beat cross frequency. ²pH = pH of Lake extender; ET: equilibration temperature after semen dilution.