Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions

Table 3

Sperm viability and CASA parameters (LSMeans ± SE) measured in budgerigar semen samples diluted in Lake extender (LE) at different pH.

Sperm variables^1	pH
	7.8	8.2	8.4
SMI (%)	86.3 ± 1.4	86.8 ± 1.1	87.5 ± 1.9
TMS (%)	6.0 ± 3.0^a	15.5 ± 2.4^b	26.2 ± 3.8^c
PMS (%)	0.3 ± 0.6^a	1.0 ± 0.4^a	3.0 ± 0.7^b
VCL (μm/s)	21.6 ± 1.0^a	25.2 ± 0.8^b	28.3 ± 1.3^c
VSL (μm/s)	5.7 ± 0.9^a	8.7 ± 0.7^b	12.6 ± 1.2^c
VAP (μm/s)	10.3 ± 1.0^a	14.3 ± 0.8^b	18.4 ± 1.3^c
LIN (%)	25.0 ± 2.4^a	33.4 ± 1.9^b	41.3 ± 3.0^c
STR (%)	55.9 ± 2.4	57.9 ± 1.9	61.5 ± 3.0
WOB (%)	44.9 ± 2.4^a	55.3 ± 1.9^b	62.7 ± 3.0^c
ALH (μm)	0.5 ± 1.2^a	1.2 ± 0.1^b	1.5 ± 0.2^b
BCF (Hz)	1.5 ± 0.5^a	2.9 ± 0.4^b	3.9 ± 0.7^b

¹SMI: sperm membrane integrity; TMS: total motile sperm; PMS: progressive motile sperm; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN = VSL/VCL × 100; STR = VSL/VAP × 100; WOB = VAP/VCL × 100; ALH: amplitude of lateral head displacement; BCF: beat cross frequency. ^a,b,cDifferent superscripts show a significant difference between treatments within row at .