Veterinary Medicine International

Research Article

Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions

Table 4

Sperm viability and CASA parameters (LSMeans ± SE) measured in budgerigar semen samples diluted in Lake extender (LE) and equilibrated at 25°C and 4°C.


Sperm variables¹	ET²
Sperm variables¹	25°C	4°C

SMI (%)	86.6 ± 1.5	87.1 ± 0.9
TMS (%)	11.3 ± 3.3^a	20.5 ± 1.9^b
PMS (%)	0.9 ± 0.69	1.9 ± 0.36
VCL (μm/s)	23.5 ± 1.1^a	26.6 ± 0.6^b
VSL (μm/s)	6.6 ± 1.0^a	11.4 ± 0.6^b
VAP (μm/s)	11.9 ± 1.19^a	16.7 ± 0.6^b
LIN (%)	26.2 ± 2.6^a	40.3 ± 1.5^b
STR (%)	50.8 ± 2.6^a	66.1 ± 1.5^b
WOB (%)	44.6 ± 2.6^a	60.0 ± 1.5^b
ALH (μm)	0.5 ± 1.2^a	1.6 ± 0.1^b
BCF (Hz)	0.9 ± 0.6^a	4.7 ± 0.3^b

¹SMI: sperm membrane integrity; TMS: total motile sperm; PMS: progressive motile sperm; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN = VSL/VCL × 100; STR=VSL/VAP × 100; WOB=VAP/VCL × 100; ALH: amplitude of lateral head displacement; BCF: beat cross frequency. ²Equilibration temperature. ^a,bDifferent superscripts show a significant difference between treatments within row at .