Veterinary Medicine International

Research Article

Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions

Table 5

Sperm viability and CASA parameters (LSMeans ± SE) measured in budgerigar semen samples diluted in 8.4 pH Lake extender (LE) at different rate before quality assessment.


Sperm variables¹	Dilution rate
Sperm variables¹	1 : 3	1 : 4

SMI (%)	87.7 ± 1.3	86.5 ± 2.6
TMS (%)	29.9 ± 2.8	25.8 ± 5.3
PMS (%)	4.1 ± 0.6	3.5 ± 1.2
VCL (μm/s)	31.3 ± 0.9	33.2 ± 1.7
VSL (μm/s)	15.3 ± 1.0	18.7 ± 2.0
VAP (μm/s)	21.4 ± 1.0	24.3 ± 2.0
LIN (%)	46.9 ± 2.0	55.2 ± 3.7
STR (%)	68.6 ± 1.5^a	75.1 ± 2.8^b
WOB (%)	66.8 ± 1.5	72.6 ± 2.9
ALH (μm)	2.1 ± 0.1	2.3 ± 0.2
BCF (Hz)	5.9 ± 0.4	7.0 ± 0.7

¹SMI: sperm membrane integrity; TMS: total motile sperm; PMS: progressive motile sperm; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN = VSL/VCL × 100; STR=VSL/VAP × 100; WOB=VAP/VCL × 100; ALH: amplitude of lateral head displacement; BCF: beat cross frequency. ^a,bDifferent superscripts show a significant difference between treatments within row at .