Comparative Study of the Immune Response Induced by an Argentinian Classical Strain of IBDV in Two Chicken Breeds

Figure 4

Anti-IBDV and anti-NDV humoral immune responses. (a) Percentage of positivity (PP) of anti-IBDV antibodies at 7, 14, 21, and 28 dpi obtained with an indirect ELISA. The mean PP values ± SD are shown for each group at the different time points; data from the negative controls of infection were less than 15% (data not shown). (b) IBDV neutralizing antibody titers in IBDV-infected chickens at 28 dpi, calculated as the inverse of the last dilution showing no cytopathic effect. Log2 of the median of each group is shown and the comparisons were made using Wilcoxon–Mann–Whitney, . (c) Percentage of positivity (PP) of anti-NDV antibodies in sera collected at 7, 14, and 21 days postvaccination with NDV measured with an in-house ELISA. Data from the negative controls of NDV vaccination were less than 15% (data not shown). Different letters indicate significant differences among groups at day 21 (one-way ANOVA test and Tukey post-hoc test, ).

(a)

(b)

(c)