Activating Mutation in the Receptor Tyrosine Kinase FLT3 with Clinicopathological Relevance in Canine Mast Cell Tumors

Figure 1

Detection of FLT3 ITDs in MCT-bearing dogs. FLT3 PCR products, obtained using the primers spanning exons 14 and 15, were analysed by agarose gel electrophoresis. A single band of 500 bp was obtained in FLT3-wild type MCT cases (lanes 3, 4, and 6), while an extra-band above 500 bp was shown in mutated samples (lanes 1, 2, 5, 7, 8, 9, and 10). Lane 1 - DNA ladder (100 bp); Lane 11 - negative PCR control (water).