Activating Mutation in the Receptor Tyrosine Kinase FLT3 with Clinicopathological Relevance in Canine Mast Cell Tumors

Table 1

Forward (F) and reverse (R) primer sequences for polymerase chain reaction with corresponding annealing temperature and their amplification products used to investigate mutations.

Genes symbol and primer sequence (5′⟶3′)	Exon	Annealing temp.	Amplification product (bp)
FLT3
F: CCATTTCTGAGGGACTGC	14-15	55	500
R: GCCTTGAAACATGGCAAGC
F: TCACCTGGAATTCCTACTGAAC	20	55	308
R: TGTACTACAGCGGTTGTGGAC