Research Article

Phenotypical Identification and Toxinotyping of Clostridium perfringens Isolates from Healthy and Enteric Disease-Affected Chickens

Table 1

Oligonucleotide primer sequences and amplified PCR product sizes were used for the detection of C. perfringens and its toxin genes.

Name of bacteria and toxinsTarget genePrimers namePrimer sequences (5′-3′)PCR product size (bp)Ref

C. perfringens16S RNAClPer-1TAACCTGCCTCATAGAGT481[15]
ClPer-2TTT​CAC​ATC​CCA​CTT​AAT​C

Alpha (α)cpaCPAlphaFGCT​AAT​GTT​ACT​GCC​GTT​GA324[11]
CPAlphaRCCT​CTG​ATA​CAT​CGT​GTA​AG
Beta (β)cpbCPBetaF3GCG​AAT​ATG​CTG​AAT​CAT​CTA195
CPBetaR3GCA​GGA​ACA​TTA​GTA​TAT​CTT​C
Epsilon (ε)etxCPEpsilonFTGG​GAA​CTT​CGA​TAC​AAG​CA376
CPEpsilonR2AAC​TGC​ACT​ATA​ATT​TCC​TTT​TCC
Iota (ι)iapCPIotaF2AAT​GGT​CCT​TTA​AAT​AAT​CC272
CpIotaRTTA​GCA​AAT​GCA​CTC​ATA​TT
Beta-2 (β2)cpb2CPBeta2totalF2AAA​TAT​GAT​CCT​AAC​CAA​CAA548
CPBeta2totalRCCAAATACTCTAATYGATGC
EnterotoxincpeCPEnteroFTTC​AGT​TGG​ATT​TAC​TTC​TG485
CPEnteroRTGT​CCA​GTA​GCT​GTA​ATT​T