Research Article
Phenotypical Identification and Toxinotyping of Clostridium perfringens Isolates from Healthy and Enteric Disease-Affected Chickens
Table 1
Oligonucleotide primer sequences and amplified PCR product sizes were used for the detection of C. perfringens and its toxin genes.
| Name of bacteria and toxins | Target gene | Primers name | Primer sequences (5′-3′) | PCR product size (bp) | Ref |
| C. perfringens | 16S RNA | ClPer-1 | TAACCTGCCTCATAGAGT | 481 | [15] | ClPer-2 | TTTCACATCCCACTTAATC |
| Alpha (α) | cpa | CPAlphaF | GCTAATGTTACTGCCGTTGA | 324 | [11] | CPAlphaR | CCTCTGATACATCGTGTAAG | Beta (β) | cpb | CPBetaF3 | GCGAATATGCTGAATCATCTA | 195 | CPBetaR3 | GCAGGAACATTAGTATATCTTC | Epsilon (ε) | etx | CPEpsilonF | TGGGAACTTCGATACAAGCA | 376 | CPEpsilonR2 | AACTGCACTATAATTTCCTTTTCC | Iota (ι) | iap | CPIotaF2 | AATGGTCCTTTAAATAATCC | 272 | CpIotaR | TTAGCAAATGCACTCATATT | Beta-2 (β2) | cpb2 | CPBeta2totalF2 | AAATATGATCCTAACCAACAA | 548 | CPBeta2totalR | CCAAATACTCTAATYGATGC | Enterotoxin | cpe | CPEnteroF | TTCAGTTGGATTTACTTCTG | 485 | CPEnteroR | TGTCCAGTAGCTGTAATTT |
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