Morphology, Biochemical, and Molecular Characterization of Pasteurella multocida Causing Hemorrhagic Septicemia in Indonesia

Table 2

PCR primer sets used in this study.

Primer name	Sequence (5′ to 3′)	PCR program	Reference
PM23F1	GGC​TGG​GAA​GCC​AAA​TCA​AAG	98°C 2.5 min, 30 × (94°C 1 min, 50°C 1 min, and 72°C 1 min), 72°C 10 min	[24]
PM23R2	CGA​GGG​ACT​ACA​ATT​ACT​GTA​A
ompH-F	ACT​ATG​AAA​AAG​ACA​ATC​GTA​G	94°C 5 min, 35 × (94°C 15 s, 55°C 1 min, and 72°C 1 min), 72°C 10 min	[25]
ompH-R	GAT​CCA​TTC​CTT​GCA​ACA​TAT​T
M13	GAGGGTGGCGGTTCT	95°C 3 min, 30 × (95°C 30 s, 44°C 58 s, and 72°C 70 s), 72°C 7 min	[26]