Research Article

Cultivable Fungi from Amazon River Dolphins Engaged in Wildlife Ecotourism in the Anavilhanas National Park, Brazil

Table 1

Analysis of aerobic fungal colony-forming units (CFUs) and species identification via PCR sequencing of ITS and 28S regions and micromorphology in the skin and oral cavity of Amazon river dolphins (Inia geoffrensis).

SampleColony forming unit/100 µL sample
SabouraudChromagar candidaNiger seedBrain heart infusion
(Bacteria CFU)

Dolphin 1: “Curumim”Oral swab001 (Exophiala dermatitidis)0 (>10)
Skin swab0000 (>10)
Skin scraping0000 (>10)

Dolphin 2:“Priscila”Oral swab0000 (>10)
Skin swab0000 (>10)
Skin scraping0000 (>10)

Dolphin 3: “Chico”Oral swab03 (Trichosporon montevideense)00 (>100)
Skin swab0000 (>100)
Skin scraping6 (Toxicocladosporium irritans)01 (unidentified)0 (>100)

Water from the collection site50 (Rhodotorula mucilaginosa)2 (Exophiala dermatitidis)1 (Nectria pseudotrichia)0 (>100)
1 (Penicillium citrinum)
1 (Fomitopsis meliae)
7 (Exophiala dermatitidis)

Water from the city harbor>100 (Candida spencermartinsiae)1 (Penicillium chermesinum)00 (>100)

Note. CFU (colony forming unit) denotes viable fungal colonies per 100 milliliter swab. Samples were collected from the oral cavities and skin of Amazon River dolphins identified by names given by the local community in the Anavilhanas National Park, Novo Airão–AM, Brazil. The agar types used were SAB (Sabouraud agar, Difco), CHRO (Chomagar Candida Bento Dickson), BHI (brain heart infusion agar, Difco), and NSA (niger agar). The samples are being presented with the “names given to the dolphins” by the community (Chico, Priscila, and Curumim).