Comparing Intradermal (ID) Rabies Vaccination with Conventional IM Regimen on Humoral Response of New Zealand White Rabbits for the Production of Animal-Derived Polyclonal Antibodies

Figure 3

Standard curve for calculating the quantitative titer of antirabies antibodies. By diluting measurement standards, the standard curve was developed. The serum titer values were determined by comparing the OD values for the unknown sample with those of the positive controls. The quantified sera titer was indicated by the equivalent units per milliliter (EU/ml), and a direct reading on the standard curve correlated to this value. Titer values were determined using the given conversion software tool. At titer values of 4, 0.5–4, 0.125–0.5, and 0.125 EU/ml, the results were classified as high seroconversion, sufficient seroconversion, insufficient seroconversion, and undetectable seroconversion.