Molecular Detection and Characterization of Hemotropic Mycoplasma in Assamese Macaques (Macaca assamensis) of Northern Thailand
Table 1
The oligonucleotide sequences, thermal cycling conditions used in the nested PCR assay for hemoplasma detection in Assamese macaques in Chiang Rai province, Thailand.
Primers
Oligonucleotide sequences (5′ ⟶ 3′)
Target gene
Thermal cycling conditions
Amplicon size (bp)
V1-F V9-R
AGAGTTTGATCCTGGCTCAG GNTACCTTGTTACGACTT
16S rRNA
95° (5 m); X40 [95° (1 m), 50° (1 m) and 72° (1 m)]; 72° (10 m)
1,400
V3-F V6-R
ACTCCTACGGGAGGCAGCAG CGACAGCCATGCANCACCT
16S rRNA
95° (5 m); X 45 [95° (1 m), 55° (45 s) and 72° (45 s)]; 72° (10 m)
