Impact of the Antioxidant Hydroxytyrosol on the Quality of Post-Thawed Stallion Semen

Table 1

The effect of adding HT at different concentrations on motility, acrosome integrity, HOST, and defect of the morphology in cooled stallion semen.

Parameters	Control	Hydroxytyrosol (μg/ml)
		1.25	2.50	5.00	10.00
TM (%)	80.71 ± 3.84^a	78.33 ± 4.40^a	81.00 ± 3.31^a	80.00 ± 3.65^a	65.00 ± 2.88^b
PM (%)	25.66 ± 5.35	29.50 ± 7.13	17.06 ± 3.74	17.05 ± 5.30	12.25 ± 4.95
VCL (μm/s)	90.27 ± 1.41	91.93 ± 0.74	91.13 ± 0.89	89.85 ± 0.77	91.12 ± 0.02
VSL (μm/s)	23.17 ± 0.67	25.03 ± 0.83	24.52 ± 0.70	24.85 ± 0.95	24.10 ± 0.40
VAP (μm/s)	46.10 ± 0.76	46.56 ± 0.66	46.17 ± 0.94	46.47 ± 0.79	46.25 ± 0.75
LIN (%)	27.31 ± 0.64	27.65 ± 1.08	27.18 ± 0.72	28.27 ± 1.03	26.85 ± 0.25
STR (%)	53.14 ± 1.00	53.80 ± 1.49	52.94 ± 0.92	53.60 ± 1.26	52.40 ± 1.80
WOB (%)	52.30 ± 0.45	52.40 ± 1.27	51.33 ± 0.78	52.44 ± 0.92	51.27 ± 1.27
Normal morphology (%)	83.77 ± 1.41	81.43 ± 1.84	78.36 ± 2.94	78.39 ± 2.47	79.10 ± 2.09
Major abnormalities (%)	9.99 ± 1.58^a	7.89 ± 1.19^b	8.70 ± 2.14^b	9.11 ± 1.84^b	6.80 ± 1.69^b
Minor abnormalities (%)	6.97 ± 2.03	10.65 ± 1.58	12.93 ± 2.73	12.49 ± 2.49	14.09 ± 2.16
HOST (%)	74.96 ± 3.45	73.00 ± 2.20	69.30 ± 4.41	70.10 ± 3.22	66.39 ± 4.36
Acrosome integrity (%)	89.03 ± 1.28	92.46 ± 1.43	90.60 ± 1.98	90.63 ± 1.90	90.86 ± 0.13

^a,b,abValues with different superscripts in the same row among groups showed significant differences at , ANOVA.