Characterisation of the Endophytic and Rhizospheric Bacillus licheniformis Strains Isolated from Sweet Potato with Plant Growth-Promoting and Yield Enhancing Potential

Table 1

Primers and temperature parameters used for polymerase chain reactions.

Primers (5′−3′)	PCR parameters	References
16S rRNA gene		[23]
Eub 341-F	95°C, 2 min (1 cycle)
CCTACGGGAGGCAGCAG	95°C, 30 s; 57°C, 45 s
Eub 1060-R	72°C, 1 min (32 cycles)
CGACACGAGCTGACGACA	72°C, 7 min (1 cycle)
DNA gyrase alpha subunit gene		[22]
gyrA-F	95°C, 5 min (1 cycle)
CAGTCAGGAAATGCGTACGTCCTT	94°C, 30 s; 50°C, 45 s
gyrA-R	94°C, 30 s (30 cycles)
CAAGGTAATGCTCCAGGCATTGCT	72°C, 2 min (1 cycle)

The DNA sequences were submitted to NCBI GenBank (accession numbers OP620082-88).