Therapeutic Effect of Hydrodynamics-Based Delivery of Matrix Metalloproteinase-13 Gene on Thioacetamide-Induced Liver Fibrosis in Rats

Figure 2

Delivery of MMP13-carrying plasmid into hepatocytes. (a) Time course of intravascular pressure during hydrodynamics-based gene delivery. (b) Electrophoresis of PCR products targeting AmpR on pBGI-MMP13. The plasmid of pBGI-MMP13 was analyzed as positive control. The expected band of AmpR is 581 bp. (c) Molar concentrations of AmpR on pBGI-MMP13. (d) Electrophoresis of RT-PCR amplicons targeting mRNA of MMP13. The expected band of MMP13 is 97 bp. (e) Molar concentrations of PCR amplicons targeting MMP13. (f) Relative quantitation of real-time PCR for MMP13 normalized to that of Gapdh for each sample. Values are expressed as (–5). Mann–Whitney test. n.s.: not significant. (g) Serum MMP13 concentration 2 weeks after the gene delivery. The values are expressed as (–5). Ctrl: no TAA treatment; T12w: fed TAA for 12 weeks; TOO: fed TAA for 12 weeks, sham procedure, following water feeding; TMO: fed TAA for 12 weeks, MMP13 delivery, following water feeding; TOT: fed TAA for 12 weeks, sham procedure, following TAA feeding; TMT: fed TAA for 12 weeks, MMP13 delivery, following TAA feeding.

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