Therapeutic Effect of Hydrodynamics-Based Delivery of Matrix Metalloproteinase-13 Gene on Thioacetamide-Induced Liver Fibrosis in Rats

Figure 3

Regressive and suppressive effect of MMP13 gene delivery on TAA-induced rat liver fibrosis. (a) Representative images of Sirius red staining of control, T12w, TOO, and TMO. The Ctrl group was not exposed to TAA. (b) Percentage of fibrotic area. (c) Quantification of hydroxyproline content. Values are expressed as (–5). Mann–Whitney test. . (d) Representative images of Sirius red staining of control, T12w, TOT, and TMT. The Ctrl group was not exposed to TAA. (e) Percentage of fibrotic area. (f) Quantification of hydroxyproline content. Values are expressed as (–5). Mann–Whitney test. . Ctrl: no TAA treatment; T12w: fed TAA for 12 weeks; TOO: fed TAA for 12 weeks, sham procedure, following water feeding; TMO: fed TAA for 12 weeks, MMP13 delivery, following water feeding; TOT: fed TAA for 12 weeks, sham procedure, following TAA feeding; TMT: fed TAA for 12 weeks, MMP13 delivery, following TAA feeding.

(a)

(b)

(c)

(d)

(e)

(f)